The 2nd Battalion, 4th Aviation Regiment was first constituted in the Regular Army on 21 August 1963 as Company B, 4th Aviation Battalion, an element of 4th Infantry Division. The unit was activated on 1 October 1963, at Fort Lewis, Washington.
The unit was deployed to the Republic of Vietnam and saw extensive action in the conflict there. The unit had campaign participation credit for the Counteroffensive Phase II, Counteroffensive Phase III, Tet Counteroffensive, Counteroffensive Phase IV, Counteroffensive Phase V, Counteroffensive Phase VI, Tet 69/Counteroffensive, Summer-Fall 1969, Winter-Spring 1970, Sanctuary Counteroffensive, and Counteroffensive Phase VII. In addition to these campaigns, the unit earned the Meritorious Unit Commendation (streamer embroidered Vietnam 1967-1968) and was awarded 2 Republic of Vietnam Crosses of Gallantry with Palm (streamers embroidered Vietnam 1966-1969 and Vietnam 1969-1970). The unit also received the Republic of Vietnam Civil Action Honor Medal, First Class (streamer embroidered Vietnam 1969-1970).
Following participation in the Vietnam conflict, the unit was inactivated on 4 December 1970, at Fort Lewis, Washington. After a 12 year absence, the unit was activated again on 17 March 1982, at Fort Carson, Colorado. It was reorganized and redesignated on 16 August 1987 as Headquarters and Headquarters Company, 2nd Battalion, 4th Aviation, an element of the 4th Infantry Division. Its organic elements were concurrently constituted and activated. It was also concurrently relieved from assignment to the 4th Infantry Division and reassigned to the 8th Infantry Division. The Battalion was inactivated on 15 November 1990 in Germany and was relieved from assignment to the 8th Infantry Division.